Int. J. Biol. Sci. 2007
International Journal of Biological Sciences
ISSN 1449-2288
www.biolsci.org 2007 3(2):85-90
© Ivyspring International Publisher.
A complete mitochondrial genome sequence of Asian black bear Sichuan subspecies (Ursus thibetanus mupinensis)
Wan-ru Hou1, Yu Chen1, Xia Wu1, Jin-chu Hu1, Zheng-song Peng1, Jung Yang1, Zong-xiang Tang2, Cai-Quan Zhou1, Yu-ming Li3, Shi-kui Yang4, Yu-jie Du1, Ling-lu Kong4, Zheng-long Ren2, Huai-yu Zhang2, Su-rong Shuai2
1.College of Life Science, China West Normal University, Nanchong 637002, China
2. Sichuan Agricultural University, Yaan 625014, China
3. Nanchong Agricultural Science Research Institute, Nanchong 637000, China
4. Sichuan Province Traditional Chinese Medicinal Materials Company Dujiangyan Raising Deer Field, Dujiangyan 611800, China
Correspondence to: Wan-ru Hou, College of Life Science, China West Normal University, 44# Yuying Road, 637002, Nanchong, China.Phone/Fax: +86-0817-2314311. E-mail: hwr168@yahoo.com.cn.
We obtained the complete mitochondrial genome of U.thibetanus mupinensis by DNA sequencing based on thePCR fragments of 18 primers we designed. The results indicate that the mtDNA is 16 868 bp in size, encodes 13protein genes, 22 tRNA genes, and 2 rRNA genes, with an overall H-strand base composition of 31.2% A, 25.4%C, 15.5% G and 27.9% T. The sequence of the control region (CR) located between tRNA-Pro and tRNA-Phe is1422 bp in size, consists of 8.43% of the whole genome, GC content is 51.9% and has a 6bp tandem repeat and two 10bp tandem repeats identified by using the Tandem Repeats Finder. U. thibetanus mupinensis mitochon-drial genome shares high similarity with those of three other Ursidae: U. americanus (91.46%), U. arctos (89.25%)and U. maritimus (87.66%).
Key words: Ursus thibetanus mupinensis; mitochondrial genomes; sequencing; sequence analysis
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