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운영자

2005-06-08 04:48:01 | Hit : 31721 | Vote : 12300

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[자료] Isolation and characterization of tetramicrosatellite DNA

Molecular Ecology Notes (2005)5 ,314 –316

doi:10.1111/j.1471-8286.2005.00912.x

Blackwell Publishing,Ltd.

PRIMER NOTE
Isolation and characterization of tetramicrosatellite DNA
markers in the Eurasian otter (Lutra lutra )

CHUAN-CHIN HUANG,*YU-CHENG HSU,*LING-LING LEE*and SHOU-HSIEN LI †
*Institute of Ecology and Evolutionary Biology,National Taiwan University,Taipei,Taiwan 106,ROC,†Department of Life Science,
National Taiwan Normal University,Taipei Taiwan 106,ROC

Abstract

Eight new tetramicrosatellite loci for Eurasian otter (Lutra lutra )were designed.Polymorphism of these eight loci in 29 otter individuals was tested.The results indicated that the allele numbers of each of loci ranged from three to five and the observed heterozygosity from 0.483 to 0.828.These new loci can be useful for population genetic research on otters and help improve the resolution of individual identification using noninvasive method.

Keywords :enrichment,Eurasian otter,Lutra lutra ,microsatellite

Received 4 November 2004;revision accepted 17 December 2004

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