::: Welcome to Conservation Genome Resource Bank for Korean Wildlife :::
 
17_c.gif ¹®ÇåÁ¤º¸
17_c.gif ¾ß»ýµ¿¹°°ü·Ã ÀÚ·á ¹× ¼Ò½Ä
17_c.gif Á¾Á¤º¸
17_c.gif º¸ÀüÀ¯ÀüÇÐ/º¸Àü»ý¹°ÇÐ ÀÚ·á
17_c.gif ¾ß»ýµ¿¹°ÀÇÇÐ ¼Ò½Ä ¹× ÀÚ·á
  - õ¿¬±â³ä¹°ÀÇ ´ë»ó
sound.gif °¶·¯¸®
sound.gif ÀÚÀ¯°Ô½ÃÆÇ (¿¾³¯ °Ô½ÃÆÇ)
sound.gif °ü·Ã»çÀÌÆ®
sound.gif ÀÚ·á½Ç
sound.gif Ã£¾Æ¿À½Ã´Â ±æ
º¸ÀüÀ¯ÀüÇÐ/º¸Àü»ý¹°ÇÐ ÀÚ·á

View Article
Name
  ¿î¿µÀÚ 2005-03-28 11:55:05 | Hit : 25836 | Vote : 7932
Subject   [ÀÚ·á] Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers
Á¦¸ñ: Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers

Thomas Jensen 1 *, Flavia M. Pernasetti 2, Barbara Durrant 1

1Center for the Reproduction of Endangered Species, Zoological Society of San Diego, San Diego, California
2University of California-San Diego, San Diego, California

email: Thomas Jensen (tjensen@sandiegozoo.org)

*Correspondence to Thomas Jensen, Zoological Society of San Diego, P.O. Box 120551, San Diego, CA 92112-0551

setDOI("ADOI=10.1002/zoo.10101") Funded by:
NOJ
Armstrong-McDonald Foundations

Keywords: molecular bird sexing • CHD gene • Chelex DNA isolation


Abstract

The ability to rapidly and reliably determine the sex of birds is very important for successful captive-bird breeding programs, as well as for field research. Visual inspection of adult birds is sufficient for sexually dimorphic species, but nestlings and monomorphic species are difficult, if not impossible, to sex by sight only. A method for rapid extraction of gDNA from blood, shell-membrane blood vessels, and fully grown feathers, using Chelex, and the PCR conditions for determination of sex-specific bands in 47 species (39 genera, 21 families, and 10 orders) are described. The PCR primers used amplify a length of DNA spanning an intron in the CHD-1 gene, which is present on both the W and Z chromosomes. The intron differs in size between the two sex chromosomes, resulting in PCR products that separate into two bands for females and a single band for males in most avian species (except ratites). Because this simple technique uses Chelex, a rapid gDNA isolation protocol, and sets of PCR primers independent of restriction enzyme digestion, birds can be accurately sexed within 5 hr of sample collection. Zoo Biol 22:561-571, 2003. © 2003 Wiley-Liss, Inc.

--------------------------------------------------------------------------------

Received: 26 July 2002; Accepted: 29 November 2002

Àüü ¹®¼­ º¸±â : ¹Ù·Î°¡±â
 Prev   [ÀÚ·á] Stereotypic behavior in Asiatic black and Malayan sun bears
¿î¿µÀÚ
  2005/03/28 
 Next   [¼Ò½Ä] ÄÚ¸£½ÃÄ« °íÀ¯Á¾ ¾ß»ý¾ç º¸È£ ÇÁ·ÎÁ§Æ®
¿î¿µÀÚ
  2005/03/20 


Copyright 1999-2024 Zeroboard / skin by daerew
151-742 ¼­¿ïƯº°½Ã °ü¾Ç±¸ ½Å¸²9µ¿ »ê56-1 ¼­¿ï´ëÇб³ ¼öÀÇ°ú´ëÇÐ 85µ¿ 802È£
Tel 02-888-2744, Fax 02-888-2754, E-mail cgrb@cgrb.org

Copyright © 2002-2004 CGRB All Rights Reserved