::: Welcome to Conservation Genome Resource Bank for Korean Wildlife :::
 
17_c.gif ¹®ÇåÁ¤º¸
17_c.gif ¾ß»ýµ¿¹°°ü·Ã ÀÚ·á ¹× ¼Ò½Ä
17_c.gif Á¾Á¤º¸
17_c.gif º¸ÀüÀ¯ÀüÇÐ/º¸Àü»ý¹°ÇÐ ÀÚ·á
17_c.gif ¾ß»ýµ¿¹°ÀÇÇÐ ¼Ò½Ä ¹× ÀÚ·á
  - õ¿¬±â³ä¹°ÀÇ ´ë»ó
sound.gif °¶·¯¸®
sound.gif ÀÚÀ¯°Ô½ÃÆÇ (¿¾³¯ °Ô½ÃÆÇ)
sound.gif °ü·Ã»çÀÌÆ®
sound.gif ÀÚ·á½Ç
sound.gif Ã£¾Æ¿À½Ã´Â ±æ
º¸ÀüÀ¯ÀüÇÐ/º¸Àü»ý¹°ÇÐ ÀÚ·á

View Article
Name
  ¿î¿µÀÚ 2006-06-07 10:26:24 | Hit : 24846 | Vote : 7836
Subject   [ÀÚ·á] An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding.
Philos Trans R Soc Lond B Biol Sci. 2005 Oct 29;360(1462):1945-58. Related Articles, Links  


An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding.

De Ley P, De Ley IT, Morris K, Abebe E, Mundo-Ocampo M, Yoder M, Heras J, Waumann D, Rocha-Olivares A, Jay Burr AH, Baldwin JG, Thomas WK.

Department of Nematology, University of California, Riverside, CA 92521, USA. paul.deley@ucr.edu

Molecular surveys of meiofaunal diversity face some interesting methodological challenges when it comes to interstitial nematodes from soils and sediments. Morphology-based surveys are greatly limited in processing speed, while barcoding approaches for nematodes are hampered by difficulties of matching sequence data with traditional taxonomy. Intermediate technology is needed to bridge the gap between both approaches. An example of such technology is video capture and editing microscopy, which consists of the recording of taxonomically informative multifocal series of microscopy images as digital video clips. The integration of multifocal imaging with sequence analysis of the D2D3 region of large subunit (LSU) rDNA is illustrated here in the context of a combined morphological and barcode sequencing survey of marine nematodes from Baja California and California. The resulting video clips and sequence data are made available online in the database NemATOL (http://nematol.unh.edu/). Analyses of 37 barcoded nematodes suggest that these represent at least 32 species, none of which matches available D2D3 sequences in public databases. The recorded multifocal vouchers allowed us to identify most specimens to genus, and will be used to match specimens with subsequent species identifications and descriptions of preserved specimens. Like molecular barcodes, multifocal voucher archives are part of a wider effort at structuring and changing the process of biodiversity discovery. We argue that data-rich surveys and phylogenetic tools for analysis of barcode sequences are an essential component of the exploration of phyla with a high fraction of undiscovered species. Our methods are also directly applicable to other meiofauna such as for example gastrotrichs and tardigrades.

PMID: 16214752 [PubMed - indexed for MEDLINE]


http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16214752&dopt=Abstract
 Prev   [ÀÚ·á] Nematode molecular diagnostics: from bands to barcodes.
¿î¿µÀÚ
  2006/06/07 
 Next   [ÀÚ·á] DNA fingerprinting of captive breeding pairs of lesser white-fronted geese (Anser erythropus) with unknown pedigrees.
¿î¿µÀÚ
  2006/05/29 


Copyright 1999-2024 Zeroboard / skin by daerew
151-742 ¼­¿ïƯº°½Ã °ü¾Ç±¸ ½Å¸²9µ¿ »ê56-1 ¼­¿ï´ëÇб³ ¼öÀÇ°ú´ëÇÐ 85µ¿ 802È£
Tel 02-888-2744, Fax 02-888-2754, E-mail cgrb@cgrb.org

Copyright © 2002-2004 CGRB All Rights Reserved