Molecular Ecology Notes
Volume 4 Issue 1 Page 56 - March 2004
doi:10.1046/j.1471-8286.2003.00564.x
Amanda S. Beheler, Jennifer A. Fike, Lisa M. Murfitt, Olin E. Rhodes Jr, Thomas S Serfass (2004)
PRIMER NOTE
Development of polymorphic microsatellite loci for North American river otters (Lontra canadensis) and amplification in related Mustelids
Abstract
In order to complement ecological research on native and reintroduced populations of the North American river otter (Lontra canadensis), we developed 10 polymorphic microsatellite loci that would permit us to evaluate population genetic structure and conduct fecal DNA analyses. The number of alleles per locus ranged from 3 to 14 and observed heterozygosity ranged from 0.23 to 0.68. Preliminary screening revealed that primers were polymorphic in a subset of other Mustelids.
Amanda S. Beheler**Department of Forestry and Natural Resources, 1159 Forestry Building, Purdue University, West Lafayette, Indiana, 47907, USA; Correspondence: Amanda Beheler. Fax: + 1 (765) 496 2422; E-mail: a-allen@fnr.purdue.edu, Jennifer A. Fike**Department of Forestry and Natural Resources, 1159 Forestry Building, Purdue University, West Lafayette, Indiana, 47907, USA; , Lisa M. Murfitt**Department of Forestry and Natural Resources, 1159 Forestry Building, Purdue University, West Lafayette, Indiana, 47907, USA; , Olin E. Rhodes Jr**Department of Forestry and Natural Resources, 1159 Forestry Building, Purdue University, West Lafayette, Indiana, 47907, USA; and Thomas S. Serfass††Department of Biology, Frostburg State University, Frostburg, Maryland, 21532, USA*Department of Forestry and Natural Resources, 1159 Forestry Building, Purdue University, West Lafayette, Indiana, 47907, USA; †Department of Biology, Frostburg State University, Frostburg, Maryland, 21532, USA
Correspondence: Amanda Beheler. Fax: + 1 (765) 496 2422; E-mail: a-allen@fnr.purdue.edu
http://www.blackwell-synergy.com/links/doi/10.1046/j.1471-8286.2003.00564.x/abs/
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