::: Welcome to Conservation Genome Resource Bank for Korean Wildlife :::
17_c.gif 문헌정보
17_c.gif 야생동물관련 자료 및 소식
17_c.gif 종정보
17_c.gif 보전유전학/보전생물학 자료
17_c.gif 야생동물의학 소식 및 자료
  - 천연기념물의 대상
sound.gif 갤러리
sound.gif 자유게시판 (옛날 게시판)
sound.gif 관련사이트
sound.gif 자료실
sound.gif 찾아오시는 길
보전유전학/보전생물학 자료

View Article
  운영자 2006-03-27 13:21:14 | Hit : 26950 | Vote : 8655
Subject   [자료] Mitochondrial DNA variable number tandem repeats (VNTRs): utility and problems in molecular ecology.
Mol Ecol. 1998 Nov;7(11):1441-55. Related Articles, Links  

Mitochondrial DNA variable number tandem repeats (VNTRs): utility and problems in molecular ecology.

Lunt DH, Whipple LE, Hyman BC.

Department of Biological Sciences, University of Hull, UK. d.h.lunt@biosci.hull.ac.uk

Analysis of mitochondrial (mt)DNA size polymorphism in the form of variable number tandem repeats (mtVNTRs) has become an increasingly popular methodology for addressing questions in molecular ecology. When detected by PCR, mtVNTR analysis can provide a sensitive, rapid, and cost-effective measure of genetic variability that may be exploited in studies of population differentiation and biogeography. Despite the emergence of this approach, there has been little critical evaluation of its success or utility as a practical tool. In this review, we identify problematic methodological, theoretical and interpretive factors that can influence the utility of mtVNTR analysis. The reliability of the procedure is considered in terms of both detection of alleles and scoring of intra-individual allele frequencies. While many of the potential technical problems of the technique do not raise serious practical concerns, this rapid and sensitive methodology is seriously compromised by the difficulty of reliably assessing allele frequencies, of assaying only germline tissue, and in our ignorance of the mechanisms generating mtVNTR diversity. Thus, although there is a considerable potential for mtVNTR pilot studies to assess genetic diversity, the utility of the technique to resolve broader questions in molecular ecology should be treated cautiously until such a time as the system is better understood.

Publication Types:

PMID: 9819900 [PubMed - indexed for MEDLINE]

 Prev   [자료] Nuclear DNA analyses in genetic studies of populations: practice, problems and prospects.

Copyright 1999-2024 Zeroboard / skin by daerew
151-742 서울특별시 관악구 신림9동 산56-1 서울대학교 수의과대학 85동 802호
Tel 02-888-2744, Fax 02-888-2754, E-mail cgrb@cgrb.org

Copyright © 2002-2004 CGRB All Rights Reserved