::: Welcome to Conservation Genome Resource Bank for Korean Wildlife :::
 
17_c.gif 문헌정보
17_c.gif 야생동물관련 자료 및 소식
17_c.gif 종정보
17_c.gif 보전유전학/보전생물학 자료
17_c.gif 야생동물의학 소식 및 자료
  - 천연기념물의 대상
sound.gif 갤러리
sound.gif 자유게시판 (옛날 게시판)
sound.gif 관련사이트
sound.gif 자료실
sound.gif 찾아오시는 길
보전유전학/보전생물학 자료

View Article
Name
  운영자 2006-02-09 14:52:27 | Hit : 16484 | Vote : 2969
Subject   [자료] Evolutionary dynamics of microsatellite DNA
Chromosoma. 2000 Sep;109(6):365-71. Related Articles, Links  


Erratum in:
Chromosoma 2001 Feb;109(8):571.

Evolutionary dynamics of microsatellite DNA.

Schlotterer C.

Institut fur Tierzucht und Genetik, Veterinarmedizinische Universitat Wien, Vienna, Austria. christian.schloetterer@vu-wien.ac.at

Within the past decade microsatellites have developed into one of the most popular genetic markers. Despite the widespread use of microsatellite analysis, an integral picture of the mutational dynamics of microsatellite DNA is just beginning to emerge. Here, I review both generally agreed and controversial results about the mutational dynamics of microsatellite DNA. Microsatellites are short DNA sequence stretches in which a motif of one to six bases is tandemly repeated. It has been known for some time that these sequences can differ in repeat number among individuals. With the advent of polymerase chain reaction (PCR) technology this property of microsatellite DNA was converted into a highly versatile genetic marker (Litt and Luty 1989; Tautz 1989; Weber and May 1989). Polymerase chain reaction products of different length can be amplified with primers flanking the variable microsatellite region. Due to the availability of high-throughput capillary sequencers or mass spectrography the sizing of alleles is no longer a bottleneck in microsatellite analysis. The almost random distribution of microsatellites and their high level of polymorphism greatly facilitated the construction of genetic maps (Dietrich et al. 1994; Dib et al. 1996) and enabled subsequent positional cloning of several genes. Almost at the same time, microsatellites were established as the marker of choice for the identification of individuals and paternity testing. The high sensitivity of PCR-based microsatellite analysis was not only of great benefit for forensics, but opened completely new research areas, such as the analysis of samples with limited DNA amounts (e.g., many social insects) or degraded DNA (e.g., feces, museum material) (Schlotterer and Pemberton 1998). More recently, microsatellite analysis has also been employed in population genetics (Goldstein and Schlotterer 1999). Compared with allozymes, microsatellites offer the advantage that, in principle, several thousand potentially polymorphic markers are available. Nevertheless, the application of microsatellites to population genetic questions requires a more detailed understanding of the mutation processes of microsatellite DNA as the evolutionary time frames covered in population genetics are often too long to allow novel microsatellite mutations to be ignored. Additional interest in the evolution of microsatellite DNA comes from the discovery that trinucleotide repeats, a special class of microsatellites, are involved in human neurodegenerative diseases (e.g., fragile X and Huntington's disease). A detailed understanding of the processes underlying microsatellite instability is therefore an important contribution toward a better understanding of these human neurodegenerative diseases.

Publication Types:
Review

MeSH Terms:
Animals
Cloning, Molecular
DNA Replication
Evolution, Molecular*
Gene Frequency
Genetics, Population
Humans
Microsatellite Repeats*
Mutation
Recombination, Genetic
Research Support, Non-U.S. Gov't

PMID: 11072791 [PubMed - indexed for MEDLINE]
 Prev   [자료] Microsatellite DNA and recent statistical methods in wildlife conservation management: applications in Alpine ibex [Capra ibex(ibex)].
운영자
  2006/02/20 
 Next   [자료] Determining the source of individuals: multilocus genotyping in nonequilibrium population genetics
운영자
  2006/02/09 


Copyright 1999-2020 Zeroboard / skin by daerew
151-742 서울특별시 관악구 신림9동 산56-1 서울대학교 수의과대학 85동 802호
Tel 02-888-2744, Fax 02-888-2754, E-mail cgrb@cgrb.org

Copyright © 2002-2004 CGRB All Rights Reserved