::: Welcome to Conservation Genome Resource Bank for Korean Wildlife :::
 
17_c.gif ¹®ÇåÁ¤º¸
17_c.gif ¾ß»ýµ¿¹°°ü·Ã ÀÚ·á ¹× ¼Ò½Ä
17_c.gif Á¾Á¤º¸
17_c.gif º¸ÀüÀ¯ÀüÇÐ/º¸Àü»ý¹°ÇÐ ÀÚ·á
17_c.gif ¾ß»ýµ¿¹°ÀÇÇÐ ¼Ò½Ä ¹× ÀÚ·á
  - õ¿¬±â³ä¹°ÀÇ ´ë»ó
sound.gif °¶·¯¸®
sound.gif ÀÚÀ¯°Ô½ÃÆÇ (¿¾³¯ °Ô½ÃÆÇ)
sound.gif °ü·Ã»çÀÌÆ®
sound.gif ÀÚ·á½Ç
sound.gif Ã£¾Æ¿À½Ã´Â ±æ
º¸ÀüÀ¯ÀüÇÐ/º¸Àü»ý¹°ÇÐ ÀÚ·á

View Article
Name
  ¿î¿µÀÚ 2006-06-07 10:32:16 | Hit : 27335 | Vote : 9302
Subject   [ÀÚ·á] Molecular barcodes for soil nematode identification.
Mol Ecol. 2002 Apr;11(4):839-50. Related Articles, Links  


Molecular barcodes for soil nematode identification.

Floyd R, Abebe E, Papert A, Blaxter M.

Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK. Robin.Floyd@ed.ac.uk

Using a molecular barcode, derived from single-specimen polymerase chain reaction (PCR) and sequencing of the 5' segment of the small subunit ribosomal RNA (SSU) gene, we have developed a molecular operational taxonomic unit (MOTU) scheme for soil nematodes. Individual specimens were considered to belong to the same MOTU when the sequenced segment of 450 bases was > 99.5% identical. A Scottish upland Agrostis-Festuca grassland soil was sampled, using both culture-based and random selection methods. One hundred and sixty-six cultured isolates were sequenced, and clustered into five MOTU. From 74 randomly sampled individuals across the study site, 19 MOTU were defined. A subsequent sample of 18 individuals from a single subplot contained eight MOTU, four of which were unique to the single subplot sample. Interestingly, seven of these MOTU were not present in the culture-independent sampling. Overall, a total of 23 MOTU were defined from only 240 sequences. Many MOTU could readily be assigned to classical, morphologically defined taxonomic units using a database of SSU sequences from named nematode species. The MOTU technique allows a rapid assessment of nematode taxon diversity in soils. Correlation with a database of sequences from known species offers a route to application of the technique in ecological surveys addressing biological as well as genetic diversity.

Publication Types:
Evaluation Studies

PMID: 11972769 [PubMed - indexed for MEDLINE]


http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=11972769
 Prev   [ÀÚ·á] Universal primers and PCR of gut contents to study marine invertebrate diets.
¿î¿µÀÚ
  2006/06/07 
 Next   [ÀÚ·á] Nematode molecular diagnostics: from bands to barcodes.
¿î¿µÀÚ
  2006/06/07 


Copyright 1999-2024 Zeroboard / skin by daerew
151-742 ¼­¿ïƯº°½Ã °ü¾Ç±¸ ½Å¸²9µ¿ »ê56-1 ¼­¿ï´ëÇб³ ¼öÀÇ°ú´ëÇÐ 85µ¿ 802È£
Tel 02-888-2744, Fax 02-888-2754, E-mail cgrb@cgrb.org

Copyright © 2002-2004 CGRB All Rights Reserved