::: Welcome to Conservation Genome Resource Bank for Korean Wildlife :::
 
17_c.gif 문헌정보
17_c.gif 야생동물관련 자료 및 소식
17_c.gif 종정보
17_c.gif 보전유전학/보전생물학 자료
17_c.gif 야생동물의학 소식 및 자료
  - 천연기념물의 대상
sound.gif 갤러리
sound.gif 자유게시판 (옛날 게시판)
sound.gif 관련사이트
sound.gif 자료실
sound.gif 찾아오시는 길
보전유전학/보전생물학 자료

View Article
Name
  운영자 2005-12-27 17:11:47 | Hit : 15073 | Vote : 2743
Subject   [자료] Evaluating mixed samples as a source of error in non-invasive genetic studies using microsatellites
Mol Ecol. 2005 Jan;14(1):195-201.


Evaluating mixed samples as a source of error in non-invasive genetic studies using microsatellites.

Roon DA, Thomas ME, Kendall KC, Waits LP.

Department of Fish and Wildlife, University of Idaho, Moscow, Idaho 83844-1136, USA. roon8505@uidaho.edu

The use of noninvasive genetic sampling (NGS) for surveying wild populations is increasing rapidly. Currently, only a limited number of studies have evaluated potential biases associated with NGS. This paper evaluates the potential errors associated with analysing mixed samples drawn from multiple animals. Most NGS studies assume that mixed samples will be identified and removed during the genotyping process. We evaluated this assumption by creating 128 mixed samples of extracted DNA from brown bear (Ursus arctos) hair samples. These mixed samples were genotyped and screened for errors at six microsatellite loci according to protocols consistent with those used in other NGS studies. Five mixed samples produced acceptable genotypes after the first screening. However, all mixed samples produced multiple alleles at one or more loci, amplified as only one of the source samples, or yielded inconsistent electropherograms by the final stage of the error-checking process. These processes could potentially reduce the number of individuals observed in NGS studies, but errors should be conservative within demographic estimates. Researchers should be aware of the potential for mixed samples and carefully design gel analysis criteria and error checking protocols to detect mixed samples.

PMID: 15643963 [PubMed - indexed for MEDLINE]

 Prev   [자료] Microsatellite analysis of the spectacled bear (Tremarctos ornatus) across its range distribution.
운영자
  2005/12/27 
 Next   [자료] Severe inbreeding depression in collared flycatchers (Ficedula albicollis)
운영자
  2005/12/09 


Copyright 1999-2020 Zeroboard / skin by daerew
151-742 서울특별시 관악구 신림9동 산56-1 서울대학교 수의과대학 85동 802호
Tel 02-888-2744, Fax 02-888-2754, E-mail cgrb@cgrb.org

Copyright © 2002-2004 CGRB All Rights Reserved